Pregled bibliografske jedinice broj: 785724
Comparative analysis of cisplatin treatment in in vitro 3D lung microtissue and monolayer cell cultures
Comparative analysis of cisplatin treatment in in vitro 3D lung microtissue and monolayer cell cultures // European respiratory Journal, Volume 46, Supplement 59
Amsterdam, Nizozemska, 2015. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 785724 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comparative analysis of cisplatin treatment in in vitro 3D lung microtissue and monolayer cell cultures
Autori
Kiss, Edit ; Avdičević, Monika ; Ernszt, David ; Pongracz, E. Judit
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
European respiratory Journal, Volume 46, Supplement 59
/ - , 2015
Skup
ERS 25th International Congress 2015
Mjesto i datum
Amsterdam, Nizozemska, 26.09.2015. - 30.09.2015
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
lung cancer; 3D cocultures; cisplatin; drug testing
Sažetak
Lung cancer is the leading cause of cancer related mortality worldwide, accounting for more than one million deaths per year. In spite of several therapeutic approaches the five-year survival rate is still very disappointing. Platinum-based chemotherapy for non–small cell lung carcinoma, which accounts for 85% of lung cancer cases, still remains a standard treatment. Cisplatin is one of the widely used Pt-drugs for treatment of lung cancer patients, although it has several drawbacks including the development of chemoresistance which lead to diminished drug effectiveness and subsequently therapeutic failure. Currently, anticancer drugs are tested in animal screening systems. In order to increase the human relevance of these drugs it is necessary to improve human in vitro cell-based models. To investigate the effect of cisplatin treatment, our research group has modelled lung adenocarcinoma in vitro assembling three dimensional models of the distal human lung. The tissue was built from human lung adenocarcinoma cell line and primary human lung fibroblasts. Comparative analysis of two and three dimensional mono- and cocultures has shown drastic differences in sensitivity to the drug. Our results indicate that differences in drug responsiveness correlate with alterations in the molecular microenvironment, including inflammatory cytokines and drug transporters. Apoptotic response to cisplatin was significantly higher in monolayer cell cultures compared to in vitro 3D lung micro tissues. As drug sensitivity showed marked differences, it is extremely important to carry on studies in 3D tissue environment to understand mechanisms of actions and monitor drug toxicity and drug efficacy.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
