Naslov
RAE-1γ ligand expression by MCMV vector promotes induction and maintenance of protective immune memory

Autori
Tršan, T., Busche, A., Abram, M., Wensveen, F., Lemmermann, N.A., Arapović, M., Babić, M., Tomić, A., Golemac, M., Brinkmann, M.M., Jäger, W., Oxenius, A., Polić, B., Krmpotić, A., Messerle, M., Jonjić, S.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
NK2013 14th Meeting of the Society for Natural Immunity / - , 2013

Skup
NK2013 14th Meeting of the Society for Natural Immunity

Mjesto i datum
Heidelberg, Njemačka, 18.09.2013. - 22.09.2013

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MCMV; CD8 T cell vaccine vector; NKG2D

Sažetak
NKG2D is lymphocyte-expressed receptor whose ligands are stress-induced proteins structurally related to MHC class I molecules. Engagement of NKG2D on natural killer (NK) cells potentiate NK cell-mediated killing of stressed cells. In addition, its engagement on NK cells leads to NK cells cytokine production, which modulates the intensity of immune response. On CD8 T cells NKG2D has a co- stimulatory role, increasing the magnitude of specific immune response. Here we demonstrate the exceptional capacity of mouse CMV (MCMV) expressing NKG2D ligand RAE-1γ to serve as highly protective vaccine-vector. We show that co-expression of RAE-1γ and foreign CD8 T cell epitope in the context of MCMV augmented epitope-specific CD8 T cell response and promoted induction of memory precursor CD8 T cells. Immunization with RAE-1γ expressing MCMV and foreign CD8 T cell epitope resulted in long-term protection against respective pathogen by means of CD8 T cells specific to vectored epitope. Moreover, MCMV expressing RAE-1γ preserved splenic dendritic cells (DCs) and hence rescued their co- stimulatory capacity. Improved antigen presentation was retained also in mice unable to cross-present antigens. Surprisingly, we found that RAE-1γ expressing MCMV vector was highly attenuated and equally protective even in NKG2D deficient mice, suggesting additional, NKG2D independent immune function of RAE1γ.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA