Naslov
Exposure of Campylobacter jejuni to Sub- inhibitory Concentrations of Pinocembrin Affects the Bacterial Virulence Potential

Autori
Kovač, Jasna ; Wu, Zuowei ; Klančnik, Anja ; Vučković, Darinka ; Bučar, Franz ; Abram, Maja ; Zhang, Qijing ; Smole Možina, Sonja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Raspor, Peter ; Ambrožič, Mateja ; Smole Možina, Sonja - Izola : Univerza na primorskem, Fakulteta za vede o zdravju, Inštitut za živila, prehrano in zdravje, 2015, 60-60

ISBN
978-961-93845-3-4

Skup
World Food Day 2015. 2nd annual conference Mediterranean Food and Nutrition.

Mjesto i datum
Portorož, Slovenija, 16.10.2015. - 17.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Campylobacter jejuni ; pinocembrin ; antimicrobial activity

Sažetak
The prevalence of antimicrobial resistance of foodborne pathogen Campylobacter jejuni increases, so new natural antimicrobials are becoming attractive alternatives. One of such compounds is pinocembrin, present in honey and therefore a part of normal healthy diet in low concentrations. First, anti-Campylobacter activity of pinocembrin was studied in vitro. Beside minimal inhibitory concentrations (MICs), the whole-genome transcriptomic response od C. jejuni to pinocembrin with the microarray approach was determined, as well as the effects on bacterial virulence potential in systemic infection in BALB/c mice model. MICs of pinocembrin were found at the median concentration of 39 mg/L on 31 Campylobacter isolates. The culture mimicking the traits of multidrug-resistant phenotype cmeR was used in further experiments. The 2-h pinocembrin pre-tretment of C. jejuni with sub-inhibitory concentration resulted in bacterial decrease in mice liver 8 days post infection as well as in decreased expression of ribosomal proteins and up-regulation of several NADH dehydrogenase sub-units and iron uptake proteins, suggesting disrupted the protein production, redox cycle and iron metabolism, which may cause disadvantage in host environment during the infection.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Biotehnologija

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