Naslov
Antitumor effects evaluation of a novel porphyrin derivative in photodynamic therapy

Autori
Li, Jian-Wei ; Wu, Zhong-Ming ; Margetić, Davor ; Zhang, L.-J. ; Chen, Zhi-Long

Izvornik
Tumor biology (1010-4283) 36 (2015), 12; 9685-9692

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
TDPP; Tumor Photosensitizer; Photodynamic therapy

Sažetak
In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5, 10, 15, 20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected ; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5- diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA