Naslov
Tissue distribution and tropism of PCV2 in first detected cases of porcine circovirus-associated disease (PCVAD) of wild boars in Serbia

Autori
Becskei, Zsolt ; Novosel, Dinko ; Polaček, Vladimir ; Cadar, Daniel ; Gavrilović, Pavle ; Savić, Mila ; Aleksić Kovačević, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
30th Meeting of the European Society of Veterinary Pathology and Annual Meeting of the European College of Veterinary Pathologists

Mjesto i datum
León, Španjolska, 05.09.2012. - 08.09.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
PCV2; wild boars; ISH

Sažetak
Introduction: Porcine circovirus type 2 (PCV2) affects both domestic swine and wild boars, causing PCVAD. There have been no reports of the presence of PCVAD in wild boars in Serbia. Materials and Methods: PCR analysis was done on spleen samples of 100 young wild boars in two hunting areas. To investigate the PCV2 distribution and tropism in PCR-positive cases, in-situ hybridization was performed on lymphoid and non-lymphoid organs, using a complementary probe detecting the non-replicative form (nPCV2) and a replicative probe detecting the replicative form (rPCV2). Results: PCR analysis proved the PCV2 infection of 24 (24/100) wild boars in both hunting areas. Microscopically, severe lymphocyte depletion, macrophage accumulation and granulomas were present in lymphoid tissue. In four PCVAD-affected wild boars a high amount of nPCV2 was detected and tropism (rPCV2) was proven for both lymphoid and non-lymphoid tissue. In the lymphoid tissue, nPCV2 genome was detected predominantly in the cytoplasm of macrophages, while rPCV2 was found occasionally in the nucleus of lymphocytes and rarely in macrophages. The nPCV2 and rPCV2 genomes were also located in epithelial cells of the renal tubules and bile ducts, hepatocytes, bronchoalveolar epithelium and enterocytes. Conclusions: The current study presents PCV2 tissue distribution and tropism in the first detected cases of PCVAD of wild boars in Serbia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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