Pregled bibliografske jedinice broj: 783189
Toxicity and antioxidant capacity of Frangula alnus Mill. bark and its active component emodin
Toxicity and antioxidant capacity of Frangula alnus Mill. bark and its active component emodin // Regulatory toxicology and pharmacology, 73 (2015), 3; 923-929 doi:10.1016/j.yrtph.2015.09.025 (međunarodna recenzija, članak, znanstveni)
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Naslov
Toxicity and antioxidant capacity of Frangula alnus Mill. bark and its active component emodin
Autori
Radić Brkanac, Sandra ; Gerić, Marko ; Gajski, Goran ; Vujčić, Valerija ; Garaj-Vrhovac, Vera ; Kremer, Dario ; Domijan, Ana-Marija
Izvornik
Regulatory toxicology and pharmacology (0273-2300) 73
(2015), 3;
923-929
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
human peripheral blood lymphocytes; cytotoxicity; genotoxicity; oxidative stress; polyphenol content
Sažetak
In the present study toxicity of Frangula alnus Mill. bark, widely used as laxative, was investigated. Human peripheral blood lymphocytes (HPBLs) were treated with F. alnus bark extract or emodin (emodin is bark component with laxative property), and cytotoxicity, genotoxicity and parameters of oxidative stress were assessed. Also, polyphenol content of bark extract and antioxidant activity of the extract and emodin measured by DPPH, ABTS and FRAP methods were examined. The bark extract (500 μg/ml) produced cell death and DNA damage, while level of ROS changed at 250 μg/ml. Emodin induced cell death and DNA damage at 150 μg/ml and 200 μg/ml, respectively, and the increase of ROS was observed at 25 μg/ml. These results suggest that both, bark extract and emodin, are cyto/genotoxic to HPBLs and that oxidative stress is involved in the mechanism of their toxicity. The results on antioxidant activity showed that, unlike emodin, bark extract possess moderate antioxidant capacity (44.6%, 46.8% and 2.25 mmol Fe2+/g measured by DPPH, ABTS and FRAP assay, respectively) that can be related to relatively high phenolic content (116.07 mg/g). However, due to toxicological properties use of F. alnus bark as well as emodin-containing preparations should be taken with caution.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Dario Kremer
(autor)
Valerija Vujčić Bok
(autor)
Marko Gerić
(autor)
Vera Garaj-Vrhovac
(autor)
Ana-Marija Domijan
(autor)
Sandra Radić Brkanac
(autor)
Goran Gajski
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE