Naslov
Early immunoreactins in peripheral blood mononuclear cells induced by Puumala virus

Autori
Cvetko Krajinović, Lidija ; Spratt, Heidi ; Tadin, Ante ; Svoboda, Petra ; Kurolt, Ivan-Christian ; Topić, Antea ; Čivljak, Rok ; Brasier, Allan R. ; Paessler, Slobodan ; Markotić, Alemka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st CroViWO - Croatian Virus Workshop - Book of Abstracts / Vanda Juranić Lisnić, Igor Jurak and Dijana Škorić - : Hrvatsko mikrobiološko društvo, 2014, 18-19

ISBN
978-953-7778-09-5

Skup
1st CroViWo - Croatian Virus Workshop - Basic and Translational Virus Research

Mjesto i datum
Rijeka, Hrvatska, 14.11.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
hantaviruses; Puumala virus; miRNA expression; immune response; hemorrhagic fever with renal syndrome

Sažetak
Hantaviruses cause hemorrhagic fever with renal syndrome (HFRS). Infections with hantaviruses are not lytic, and it is currently unknown why infections in humans cause the disease. It is considered that pathogenesis of HFRS is mainly mediated by immune response. The aim of this study was to explore the components of innate and adaptive immunity important in the early peripheral immune response as well as the possible regulatory effect of miRNA on early immunoreactions during the hantaviral infection. Based on this, biological pathways relevant for the immunopathogenesis of HFRS have also been defined. Using real-time PCR array technology, the relative expression of immune response genes and miRNA was measured in peripheral blood mononuclear cells of patients infected with Puumala virus (PUUV). The results showed suppression of the early immune response to PUUV i.e. genes coding the synthesis of pattern recognition receptors, chemokines and their receptors, cytokines, transcription factors, as well as some signalling molecules. Functional analysis showed that down regulation of the expression of described set of genes modulates inflammatory response by interfering with various cell-signalling pathways. For the first time, the biological importance of miRNA in the regulation of immune response during HFRS was shown. Changes detected at the immune response level have been associated with disease severity but not with the viral load in the blood.

Izvorni jezik
Engleski

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