Naslov
Polyphenol constituents responsible for antiaggregatory activity of propolis

Autori
Bojić, Mirza ; Antolić, Andrea ; Rimac, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program and Abstracts 24th Scientific Congress of the Austrian Pharmaceutical Society (ÖPhG) / - Beč, 2015, 64-64

Skup
24th Scientific Congress of the Austrian Pharmaceutical Society

Mjesto i datum
Beč, Austrija, 12.09.2015. - 14.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
polyphenol; propolis; antiaggregatory

Sažetak
Propolis presents one of the richest sources of polyphenols and has numerous biological activities that include antibacterial, antifungal, antiviral, antineoplastic, hepatoprotective, immunomodulating, and anti-inflammatory, etc. As we have previously shown, flavonoids influence platelet aggregation[1], thus the goal of this study was to determine phenolic acids and flavonoids in propolis that contribute the most to the antiaggregatory activity of propolis. Twenty-two ethanolic extracts were analyzed by HPLC equipped with diode array detector. Separation of polyphenols analyzed was performed on a Agilent Zorbax SBC18 column (250mm×4.6mm, particle size 5μm), using mobile phases: A-water/methanol/formic acid (93:5:2) and B-water/methanol/formic acid (3:95:2) with the following timetable (t/min, %B): (0, 20), (10, 40), (35, 50), (47, 50), (70, 80), (80, 20). Chromatograms were recorded at 270, 290, 320 and 350 nm. Platelet aggregation was analyzed in whole blood utilizing an impedance analyzer. The results of aggregation assay were expressed as the lowest concentration of flavonoid that caused a statistically significant reduction of aggregation when compared to the untreated samples. Multiple linear regression analysis and random forest was used to assess the constituents of propolis that contribute the most to the antiaggregatory activity of propolis. The most prominent constituents were total phenolic acids and content of flavonoid apigenin.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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