Naslov
INTERACTION OF SPONGE NON-METASTATIC GROUP I NME GENE/PROTEIN WITH TELOMERES

Autori
Perina, Dragutin ; Mikoč, Andreja ; Ćukušić, Andrea ; Herak Bosnar, Maja ; Ćetković Helena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
"From molecules to life and back" FEBS 3+Meeting / Dumić, Jerka ; Kovarnik, Zrinka ; Varljen, Jadranka - Rijeka : Hrvatsko Društvo za Biotehnologiju, 2012, 160-160

ISBN
978-953-95551-4-4

Skup
"From molecules to life and back" FEBS 3+Meeting

Mjesto i datum
Opatija, Hrvatska, 13.06.2012. - 16.06.2012

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Nme1; Nme2; TRF1; kinase activity; telomere

Sažetak
The Nme family, initially called nucleoside diphosphate kinases (NDPK) are evolutionarily conserved enzymes present in all three domains of life. Human Nme1 is the most studied representative of the family and the first identified metastasis suppressor. NDPK is involved in the maintenance of the cellular NTP pool, transferring the phosphate group through the histidine phosphointermedier. Interestingly, it seems that this biochemical feature of NDPK is not responsible for its antimetastatic activity. Several other biochemical functions have been assigned to this protein: histidine kinase activity, 3'-5' exonuclease and DNA cleavage activity, and transcriptional regulatory activity. The Nme family is known to participate in numerous crucial biological events such as proliferation, differentiation, development and apoptosis, as well as in adhesion, migration, and vesicular trafficking. In spite of the comprehensive scientific activity in this area it is still unclear which biochemical/biological activities are responsible for Nme's antimetastatic role. Vertebrate Nme enzymes can be separated into two evolutionarily distinct groups. In humans, Group I includes Nme1-Nme4 and Group II includes Nme5-Nme9 proteins. Nme 1 and 2 exhibit 89% amino acid (aa) sequence homology and possess many identical/similar but also some distinct properties. A good example is the Nme1/2 affinity towards telomeres and telomeric proteins in humans. Sponges (Porifera) are simple metazoans without tissues, closest to the common ancestor of all animals. They changed little during evolution and probably provide the best insight into the metazoan ancestor's genomic features. The sponge possesses one Nme Group I protein, NmeGp1Sd. Here we report new results on the metastasis suppressor gene/protein homolog from the marine sponge Suberites domuncula. Our research will broaden the knowledge on Nme1/2-telomere interactions in humans, and since the sponge homolog is an ancestral-type protein that emerged before the duplication and diversification of the Group I Nme family it represents an ideal model protein. The purpose of this study was to investigate the properties of the sponge Group I Nme gene and protein, and compare it to its human homolog in order to elucidate the evolution of the structure and function of Nme.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA