Naslov
Macrodomain protein from bacterium Streptomyces coelicolor

Autori
Lalić, Jasna ; Mikoč, Andreja ; Perina, Dragutin ; Sabljić, Igor ; Pleše, Bruna ; Imešek, Mirna ; Ćetković, Helena ; Luić, Marija ; Žaja, Roko ; Ahel, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Congress of the Croatian Society of Biochemistry and Molecular Biology “The Interplay of Biomolecules” (HDBMB2014) : book of abstracts / Katalinić, Maja ; Kovarik, Zrinka - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2014, 107-107

ISBN
978-953-95551-5-1

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology "The Interplay of Biomolecules"

Mjesto i datum
Zadar, Hrvatska, 24.09.2014. - 27.09.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
DNA damage response ; Macrodomain ; Poly(ADP‐ribose)

Sažetak
Macrodomains are evolutionary conserved structural domains that bind ADP-ribose derivatives found in proteins with diverse cellular functions. Some proteins from the macrodomain family (human MacroD1, MacroD2) can hydrolyze ADP-ribosylated substrates and therefore reverse posttranslational modification - ADP-ribosylation in which an ADP-ribose moiety from NAD+ is transferred to a target protein. ADP-ribosylation can alter the physical and chemical properties of target proteins and controls many important cellular processes. Bacteria, and Streptomyces in particular, are known to utilize protein ADP- ribosylation to control a variety of important pathways (such as morphological differentiation and antibiotic production). We have determined crystal structure and biochemically and functionally characterized a macrodomain protein form bacterium Streptomyces coelicolor. This protein is a member of an uncharacterised subfamily of macrodomain proteins. Its crystal structure revealed highly conserved macrodomain fold. This macrodomain protein is involved in the DNA damage response, while knockout strain shows a conditional effect on antibiotic production. Our results give us a first insight into the molecular basis of its action, involvement in ADP-ribosylation cycle and impact on Streptomyces metabolism.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA