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Pregled bibliografske jedinice broj: 779785

Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)

Marković, Zvjezdana; Preiner, Darko; Stupić, Domagoj; Andabaka, Željko; Maletić, Edi; Karoglan Kontić, Jasminka; Chatelet, Philippe; Engelmann, Florent
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.) // The Pannonian Plant Biotechnology Association (PPBA) - Integration of fundamental research into the practical agriculture Progress and Perspectives / Ervin Balasz, Borut Bohanec (ur.).
Ljubljana: University of Ljubljana, Biotechnical Faculty, 2015. str. 7-7 (predavanje, nije recenziran, sažetak, znanstveni)

CROSBI ID: 779785 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)

Autori
Marković, Zvjezdana ; Preiner, Darko ; Stupić, Domagoj ; Andabaka, Željko ; Maletić, Edi ; Karoglan Kontić, Jasminka ; Chatelet, Philippe ; Engelmann, Florent

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The Pannonian Plant Biotechnology Association (PPBA) - Integration of fundamental research into the practical agriculture Progress and Perspectives / Ervin Balasz, Borut Bohanec - Ljubljana : University of Ljubljana, Biotechnical Faculty, 2015, 7-7

Skup
The Pannonian Plant Biotechnology Association (PPBA) Integration of fundamental research into the practical agriculture Progress and Perspectives

Mjesto i datum
Ljubljana, Slovenija, 08.07.2015. - 10.07.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
grapevine; cryopreservation; cryotherapy; genetic stability

Sažetak
This study aimed at establishing a cryopreservation protocol for grapevine shoot tips and at testing the efficiency of cryotherapy. In vitro cultures of healthy genotypes of eight Croatian autochthonous grapevine cultivars and of virus-infected genotypes of Plavac mali observed a high genotype effect and lower reactivity of infected genotypes. A PVS2-based cryopreservation protocol established led to approximately 50% recovery with cultivar Portan and three of the four international cultivars tested. By contrast, no or very low recovery was noted with the Croatian cultivars tested. ELISA tests revealed the elimination of the GFLV virus (77.8%) in cultivar Chardonnay and the GLRaV-3 virus (100%) in cultivar Cabernet Sauvignon in cryopreserved samples, respectively. Elimination was possible before cryopreservation. Immunolocalisation revealed GFLV-infected particles in meristematic tissues and GLRaV-3-infected particles in sieve elements. Genetic stability tested with eight AFLP primer combination, observed polymorphic fragments with increasing durations of exposure to PVS2 solution.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)



POVEZANOST RADA

Ustanove:
Agronomski fakultet, Zagreb

Profili:

Avatar Url Zvjezdana Marković (autor)

Avatar Url Edi Maletić (autor)

Avatar Url Jasminka Karoglan-Kontić (autor)

Avatar Url Željko Andabaka (autor)

Avatar Url Domagoj Stupić (autor)

Avatar Url Darko Preiner (autor)

Citiraj ovu publikaciju:

Marković, Zvjezdana; Preiner, Darko; Stupić, Domagoj; Andabaka, Željko; Maletić, Edi; Karoglan Kontić, Jasminka; Chatelet, Philippe; Engelmann, Florent
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.) // The Pannonian Plant Biotechnology Association (PPBA) - Integration of fundamental research into the practical agriculture Progress and Perspectives / Ervin Balasz, Borut Bohanec (ur.).
Ljubljana: University of Ljubljana, Biotechnical Faculty, 2015. str. 7-7 (predavanje, nije recenziran, sažetak, znanstveni)
Marković, Z., Preiner, D., Stupić, D., Andabaka, Ž., Maletić, E., Karoglan Kontić, J., Chatelet, P. & Engelmann, F. (2015) Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.). U: Ervin Balasz, B. (ur.)The Pannonian Plant Biotechnology Association (PPBA) - Integration of fundamental research into the practical agriculture Progress and Perspectives.
@article{article, author = {Markovi\'{c}, Zvjezdana and Preiner, Darko and Stupi\'{c}, Domagoj and Andabaka, \v{Z}eljko and Maleti\'{c}, Edi and Karoglan Konti\'{c}, Jasminka and Chatelet, Philippe and Engelmann, Florent}, editor = {Ervin Balasz, B.}, year = {2015}, pages = {7-7}, keywords = {grapevine, cryopreservation, cryotherapy, genetic stability}, title = {Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)}, keyword = {grapevine, cryopreservation, cryotherapy, genetic stability}, publisher = {University of Ljubljana, Biotechnical Faculty}, publisherplace = {Ljubljana, Slovenija} }
@article{article, author = {Markovi\'{c}, Zvjezdana and Preiner, Darko and Stupi\'{c}, Domagoj and Andabaka, \v{Z}eljko and Maleti\'{c}, Edi and Karoglan Konti\'{c}, Jasminka and Chatelet, Philippe and Engelmann, Florent}, editor = {Ervin Balasz, B.}, year = {2015}, pages = {7-7}, keywords = {grapevine, cryopreservation, cryotherapy, genetic stability}, title = {Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)}, keyword = {grapevine, cryopreservation, cryotherapy, genetic stability}, publisher = {University of Ljubljana, Biotechnical Faculty}, publisherplace = {Ljubljana, Slovenija} }

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