Naslov
Changes in hepatic lipogenesis induced by sucrose: principal component analysis

Autori
Miljak, Katarina ; Starčević, Kristina ; Mašek, Tomislav

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstract The 6th international congress " Veterinary science and profession" / Horvatek-Tomić, Danijela ; Severin, Krešimir ; Slavica, Alen - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2015, 69-69

Skup
The 6th international congress " Veterinary science and profession"

Mjesto i datum
Zagreb, Hrvatska, 01.10.2015. - 02.10.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lipogenesis ; Sucrose ; Fatty acids ; PCA

Sažetak
This study was conducted to investigate the effect of long-term treatment with sucrose in drinking water on fatty acid profile of the liver in rats fed high-fiber diet. We used Whistar rats divided into two groups: control and experimental which received 30 % sucrose in drinking water. Liver homogenate lipids were extracted, separated by thin layer chromatography and fatty acid composition was determined by gas chromatography mass spectrometry. The treatment significantly changed the fatty acid profile of the liver. The most significant alterations were the increase of C16:0, C16:1n7 and C18:1n9 ; and the decrease of C18:0, C18:2n6, C18:3n3, C20:4n6 and C20:5n3. Summarized fatty acid profile showed a significant increase in monounsaturated fatty acids and saturated fatty acids. Observed changes were the results of several factors, of which the most important were: the increase in β-oxidation of C18:2n6 and C18:3n3, the increase in de novo lipogenesis and the changes in the activity of Δ-desaturases. Principal component analyses load plot of fatty acids shows that MUFA and C16:0 are directly opposite to C-18 and C-20 fatty acids. This suggests that increase in DNL products was at the expense of C-18 and C-20 fatty acids rather than at the expense of C-22 fatty acids (C22:5n3 and C22:6n3). Treatment with sucrose extensively changes fatty acid composition of hepatic lipids, increases de novo lipogenesis and its products as well as increases oxidative stress.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Veterinarska medicina

POVEZANOST RADA