Naslov
Trace metals in fish biominerals as environmental indicators: handheld XRF analyses

Autori
Medaković, Davorin ; Dolenec, Tadej ; Karlović, Danijel ; Vrhovnik, Petra ; Rogan Šmuc, Nastja ; Rončević, Sanda ; Pitarević-Svedružić, Lovorka ; Dolenec, Matej

Vrsta, podvrsta i kategorija rada
Poglavlja u knjigama, znanstveni

Knjiga
Biomineralization: From Fundamentals to Biomaterials & Environmental Issues

Urednik/ci
Marin, Frédéric ; Brümmer, Franz ; Checa, Antonio ; Furtos, Gabriel ; Lesci, Isidoro Giorgio ; Šiller, Lidija

Izdavač
Trans Tech Publications

Grad
Pfäffikon

Godina
2015

Raspon stranica
328-339

ISBN
978-3-03835-591-5

ISSN
1662-9795

Ključne riječi
Sparus aurata ; Diplodus sargus ; X-ray Fluorescence (XRF) ; ICP-AES elemental analysis ; X-ray diffraction ; heavy metals ; fish biominerals

Sažetak
Abstract The aim of this study was to investigate the use of physical experimental approaches for the routine determination of heavy metals in different biominerals, for bio- monitoring and ecological studies of the environment. Jawbones and selected teeth (malleolus) of a set of adult marine benthic fishes Sparus aurata and Diplodus sargus were collected from different stations along East Adriatic coast (Croatia). These samples were prepared for three different analyses: X-ray Fluorescence (XRF) ; inductively coupled plasma spectroscopy (ICP-AES) ; X-ray diffraction (XRD). The two firsts allow determining elemental composition, while the third one gives information on the mineralogical characteristics. The XRF results showed major and traces elements incorporated in the fish teeth. The concentration of zinc (Zn) expressed in mg kg -1 in the enamel - outside part of the malleolus dental surface (tooth crown) - varied widely from 77 up to 1.975 in both fish species. In the root (dentin) of the same samples, the concentration in Zn was in the range 77 - 153 mg kg -1. Sr exhibited a different concentration distribution: highest amounts were registered in the root (801 - 1.310 mg kg -1 ) and less in the enamel (476 - 1.104 mg kg -1). Multivariate statistical analysis based on Statistica 6.0. PCA (Principal Component Analysis) has been used to determine differences among elemental composition of the major and trace elements in teeth enamel (crown) and teeth dentin (roots), and in examining correlation among incorporated elements and environmental characteristics of the sampling localities. The XRD patterns of both fish jawbones and malleolus teeth showed characteristic hydroxyapatite (HAP) patterns. The HAP peaks of the fish jawbones are broadened and shifted on the higher angles, indicating that mandible and maxilla biominerals are not fully crystallized. This suggests that the fish jawbone mineralization is a dynamic and permanent process during all organism life. The content of bulk fishbone samples in selected metal elements (Ni, Co, Fe, Cu, Cr) expressed in mg kg-1 was determined by ICP-AES analysis. Higher values were obtained in the teeth than in the fish jawbones, and the element concentrations varied according to the same trend in both mineralized tissues: Fe > Cr > Cu > Ni > Co. XRF and ICP-AES showed good agreement for the determination of heavy metal pollutants. In addition, our results indicate that XRF can be used as a powerful technique for determining heavy metals content of selected biominerals, by combining cost- effectiveness, while drastically reducing analytical time.

Izvorni jezik
Engleski

Znanstvena područja
Geologija, Kemija, Biologija

POVEZANOST RADA