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Pregled bibliografske jedinice broj: 775602

Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration


Vojta, Lea; Ljuma-Skupnjak, Lana; Budimir, Ankica; Vukičević, Slobodan; Fulgosi, Hrvoje
Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration // Biotechnology reports, 7 (2015), 81-86 doi::10.1016/j.btre.2015.05.006 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 775602 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration

Autori
Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir, Ankica ; Vukičević, Slobodan ; Fulgosi, Hrvoje

Izvornik
Biotechnology reports (2215-017X) 7 (2015); 81-86

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression

Sažetak
We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin in industrial tobacco.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Zagreb,
Genera d.d.

Profili:

Avatar Url Hrvoje Fulgosi (autor)

Avatar Url Lea Vojta (autor)

Avatar Url Ankica Budimir (autor)

Avatar Url Lana Ljuma Skupnjak (autor)

Citiraj ovu publikaciju:

Vojta, Lea; Ljuma-Skupnjak, Lana; Budimir, Ankica; Vukičević, Slobodan; Fulgosi, Hrvoje
Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration // Biotechnology reports, 7 (2015), 81-86 doi::10.1016/j.btre.2015.05.006 (međunarodna recenzija, članak, znanstveni)
Vojta, L., Ljuma-Skupnjak, L., Budimir, A., Vukičević, S. & Fulgosi, H. (2015) Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration. Biotechnology reports, 7, 81-86 doi::10.1016/j.btre.2015.05.006.
@article{article, author = {Vojta, Lea and Ljuma-Skupnjak, Lana and Budimir, Ankica and Vuki\v{c}evi\'{c}, Slobodan and Fulgosi, Hrvoje}, year = {2015}, pages = {81-86}, DOI = {doi:10.1016/j.btre.2015.05.006}, keywords = {tobacco, transient expression, GM-CSF, vacuum infiltration, heterologous expression}, journal = {Biotechnology reports}, doi = {doi:10.1016/j.btre.2015.05.006}, volume = {7}, issn = {2215-017X}, title = {Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration}, keyword = {tobacco, transient expression, GM-CSF, vacuum infiltration, heterologous expression} }
@article{article, author = {Vojta, Lea and Ljuma-Skupnjak, Lana and Budimir, Ankica and Vuki\v{c}evi\'{c}, Slobodan and Fulgosi, Hrvoje}, year = {2015}, pages = {81-86}, DOI = {doi:10.1016/j.btre.2015.05.006}, keywords = {tobacco, transient expression, GM-CSF, vacuum infiltration, heterologous expression}, journal = {Biotechnology reports}, doi = {doi:10.1016/j.btre.2015.05.006}, volume = {7}, issn = {2215-017X}, title = {Rapid transient expression of human granulocyte-macrophage colony-stimulating factor in two industrial cultivars of tobacco (Nicotiana tabacum L.) by agroinfiltration}, keyword = {tobacco, transient expression, GM-CSF, vacuum infiltration, heterologous expression} }

Časopis indeksira:


  • Scopus


Uključenost u ostale bibliografske baze podataka::


  • TULSA (Petroleum Abstracts)
  • Science direct


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