Naslov
Rapid and low cost production of hGM-CSF cytokine in industrial tobacco – proof of concept

Autori
Vojta, Lea ; Ljuma-Skupnjak, Lana ; Budimir Ankica ; Vukičević Slobodan ; Fulgosi, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Global Biotechnology Congress 2015 / - Boston (MA) : Eureka Conferences Inc., 2015, 290-290

Skup
Global Biotechnology Congress 2015

Mjesto i datum
Boston (MA), Sjedinjene Američke Države, 22.07.2015. - 25.07.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Tobacco; transient expression; GM-CSF; vacuum infiltration; heterologous expression

Sažetak
We report the production of hGM-CSF protein in leaves of two industrial cultivars of tobacco by using Agrobacterium-mediated transient expression. For overexpression, two binary destination vectors, p2GW7.0 and pH7WG2.0, were evaluated. The gene hGM-CSF was tagged on the 3’ end with the His and HA-epitopes and cloned into the destination vectors. Agrobacteria containing constructs GM-CSFHis- HA/pH7WG2.0 and GM-CSF-His-HA/p2GW7.0 were used for 5- and 10-minute-long vacuum infiltration of tobacco plants. The hGM-CSF accumulation was found to be dependent on the presence of wetting agent supplemented during vacuum-agroinfiltration, duration of vacuum-agroinfiltration, and the age of the leaves. We demonstrate that industrial tobacco cultivars, with very high biomass, are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF in industrial tobacco was achieved in less than three months, opening the possibility for future applications of this approach in rapid response production of various proteins of non-plant origin.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA