Naslov
Simultaneous Determination of Phenolic Compounds in Different Matrices using Phenyl-Hexyl Stationary Phase

Autori
Tomaz, Ivana ; Maslov Luna

Izvornik
Food analytical Methods (1936-9751) 19 (2016), 2; 401-410

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Phenyl-hexyl stationary phase ; Fluorescence detection ; Phenolic compounds ; HPLC

Sažetak
A novel unique liquid chromatographic method has been developed consisting of diode array and fluorescence detection, using phenyl-hexyl stationary phase of the column, for the determination of over thirty phenolic compounds (anthocyanins, flavonols, flavan-3-ols, hydroxybenzoic acids, hydroxycinammic acids, and stilbenes). The method was validated including the following parameters: linearity, limits of detection and quantification, precision, and reproducibility. Excellent selectivity and sensitivity have been achieved. Detection levels ranging from 1.79 × 10−5 mg/L to 0.26 mg/L were obtained. New fluorescence wavelengths for detection of flavan-3-ols (λ ex = 225 and λ em = 320 nm) were more sensitive than previously published. The new optimized method showed good repeatability and reproducibility, and values for relative standard deviations (RSD) were less than 3 %. Applicability of the method was demonstrated for five different matrices, white and red wine from Vitis vinifera cultivars, apple cider, blackberry wine, and grape skin extracts, showing that method is robust and can be applied for routine analysis.

Izvorni jezik
Engleski

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