Naslov
Fluorophore-tagged Nucleobase Derivatives

Autori
Saftić, Dijana ; Žinić, Biserka ; Radić Stojković, Marijana ; Tumir, Lidija-Marija ; Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXIV. Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka / Ukić, Šime ; Bolanča, Tomislav - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2015, 148-148

ISBN
978-953-6894-54-3

Skup
XXIV. Hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 21.04.2015. - 24.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Nukleobaze; click kemija; fluorescentni konjugati
(Nucleobases; Click Chemistry; Fluorescence Conjugates)

Sažetak
Novel 1, 4-disubstituted-1, 2, 3-triazole pyrimidines were synthesized applying the copper(I)-catalysed azide–alkyne cycloaddition (CuAAC). Beside intrinsically fluorescent triazole ring (well-known pharmacophore), various substituents (originating from different azide precursors) additionally enable a wide range of applications of new derivatives. For instance, 1, 2, 3-triazole ring can noncovalently interact with DNA/RNA by hydrogen bonding and aromatic stacking interactions and spectrophotometrically report on small structural differences of DNA as well as RNA secondary structure. Novel triazole-nucleobase derivatives were prepared as amino acids with both, N- and C- enabled coupling, which allows easy and versatile incorporation into large variety of novel oligopeptides for various applications. Furthermore, triazole-nucleobase was combined with phenanthridine, later added with the aim to increase DNA/RNA affinity and contribute to the spectrophotometric recognition, thus yielding dual-fluorescence reporter conjugate.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA