Naslov
Analytical methods for determination of nutritive and toxicological aspect of selected foods

Autori
Saftić, Lara

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Odjel za biotehnologiju

Mjesto
Rijeka

Datum
14.07

Godina
2015

Stranica
71

Mentor
Malenica Staver, Mladenka ; Pleadin, Jelka

Ključne riječi
aflatoxin B1 ; ochratoxin A ; nutritional aspect ; toxicological aspect ; food labelling

Sažetak
Despite the increasing number of methods for determination of nutritive aspect, routine methods have not changed significantly over the years. New Commission Regulation (EU) No 1169/2011 determines the food information that should be disclosed to consumers, and therefore sets the need to develop cheap, simple and quick automated methods for food analysis. In this thesis, prosciutto samples from Istria and Dalmatia were tested, and some of their obligatory (protein, water and fat content) and non-obligatory (pH, ash and fatty acids content) analyses of nutritional parameters were performed. By correlating accuracy and sensitivity of obtained results with legislatively tolerated deviations, there is a need for adjustment of methods used in protein, water and ash analysis, as well as a need for determination of universal technique of sampling. Although the toxicological aspect is not printed on labels, it should be provided for products when there is a danger of food contamination. The Commission Regulation (EU) No 1881/2006 amended by the Commission Regulation (EU) No 165/2010 determines the thresholds for some food contaminants, including mycotoxins, that can have wide range of negative effects on human health. However, these mycotoxin limits are not set for meat products. In order to show the need for setting the mycotoxin threshold in this kind of products, in this research aflatoxin B1 and ochratoxin A occurrence was monitored in Istrian and Dalmatian prosciutto. The results of ELISA method showed that ochratoxin A is the dominant contaminant, which is spread among samples regardless of the production method. However, because of the fact that mycotoxin concentrations in food samples are low, there is a necessity for further validation. Thus, ELISA positive samples are additionally tested with HPLC-FLD as confirmation method whereas in the last decades an increasingly important application have very sophisticated methods such as LC-MS/MS.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA