Naslov
Case study report : Severe deficiency of memory B cells and expansion of CD3+CD8+CD16high+ T cells detected by polychromatic flow cytometry

Autori
Živković, Jelena ; Banić, Ivana ; Lokas Bulat, Sandra ; Navratil, Marta ; Kobal Mrkić, Iva ; Polančec, Denis ; Turkalj, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
30th Congress of the International Society for Advancement of Cytometry : abstracts / - , 2015, Xx-xx

Skup
Congress of the International Society for Advancement of Cytometry (30 ; 2015)

Mjesto i datum
Glasgow, Ujedinjeno Kraljevstvo, 26.06.2015. - 30.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CVID ; B cells ; flow cytometry

Sažetak
Common variable immunodeficiency (CVID) is defined as a heterogeneous group of disorders characterized by low levels of serum immunoglobulins and increased susceptibility to infections. It is considered to be the most frequent clinically symptomatic primary antibody disorder, induced by intrinsic B cell defect, T cell defect, mutations in TNF receptors or without a known genetic defect. Diagnosis should be suspected in cases of recurrent sinopulmonary infections exceeding an agespecific frequency and is based on exclusion of other causes of hypogammaglobulinemia. Due to recurrent sinopulmonary infections and previous diagnosis of immunodeficiency disorder (CVID D83.0), 17-year old boy was admitted to our hospital. Family history showed no abnormalities. He showed frequent urinary and respiratory infections from infancy and was hospitalized at the age of 9 months due to agranulocytosis, hypo IgG (1.5 g/l) and sepsis caused by Pseudomonas. From the age of 12 months he is repeatedly receiving immunoglobulin substitution therapy. Comprehensive immunophenotyping of whole blood was performed using polychromatic flow cytometry. Peripheral whole blood samples were stained with the antibodies for cell surface markers CD45, CD3, CD4, CD8, CD19, CD56 and CD16 and for the two activation markers HLA-DR and CD27. Samples were lysed and fixed with 1x FACSLysing solution (BD Biosciences, USA). Upon centrifugation, leukocyte pellets were resuspended in 1% paraformaldehyde/PBS. Acquisition of samples was performed using the Navios flow cytometer (Beckman Coulter, USA). Data was analyzed using the FlowLogicTM software package (Inivai Technologies, Australia). Severe B cell and memory B cell deficiency was detected in four consecutive immunophenotypizations. In addition, noticeable increase in relative number of CD3+CD16+ T cells was detected with expanded CD3+CD8+CD16high+ subpopulation which represents aproximately 80% of total CD3+CD16+ T cells. In the two latest immunophenotypizations the onset of NK deficiency was also observed. Suspected deficiency of the memory B cells was succesfuly confirmed for patient with CVID diagnosis using polychromatic flow cytometry. Expanded immune monitoring panel enabled detection of typical CVID immunophenotype and revealed accompained NK defficiency and expansion of CD3+CD8+CD16high+ cells. Since there are no data in literature about relationship between CVID and expansion of CD3+CD8+CD16high+ cells, further studies on CVID patients should include described approach in combination with assessment of switched memory B cells in order to get new insights into this complex immunodeficiency syndrome.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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