Naslov
Deoxynivalenol-sulfates: New conjugates occurring in planta and in human urine

Autori
Warth, Benedikt ; Fruhmann, Philipp ; Wiesenberger, Gerlinde ; Kluger, Bernhard ; Šarkanj, Bojan ; Krska, Rudolf ; Doris, Marko ; Adam, Gerhard ; Schuhmacher, Rainer.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
37'th Mycotoxin Workshop, conference abstracts / Gareis, Manfres ; Karlovsky, Petr ; Meyer, Karsten ; Gottschalk, Christoph - Bratislava, 2015, 148-148

Skup
37'th Mycotoxin Workshop

Mjesto i datum
Bratislava, Slovačka, 01.06.2015. - 03.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Deoxynivalenol-sulfates; Biomarkers; human urine; mycotoxin metabolism

Sažetak
The trichothecene mycotoxin deoxynivalenol (DON) is a common food contaminant and plays a crucial role in the development and spread of the plant disease Fusarium head blight. DON is extensively metabolized in plants, animals, and humans resulting in diverse chemical and toxicological properties of the metabolites. The DON-sulfates are a so far less investigated group of DON conjugates. Recently, two DON- sulfate isomers were chemically synthesized to study their natural occurrence and toxicological potential [1]. The synthesized and NMR confirmed substances were used as reference standards to develop an LC-MS/MS based method for their quantification in methanolic wheat extracts [2]. Both isomers, namely the DON-3-sulfate and the DON-15- sulfate, were determined in wheat plants artificially treated with DON, demonstrating the plants potential to form these metabolites. Furthermore, it was shown that both isomers can be regarded as detoxification products of DON in wheat. Besides its occurrence in planta, a DON-sulfate conjugate was also identified in human urine samples. The tested samples have been previously shown to contain extremely high concentrations of DON and its major urinary conjugates DON-3-glucuronide and DON-15- glucuronide [3]. Though the absolute concentration of the DON-sulfate was lower than those of DON-glucuronides, this novel human excretion mechanism warrants more detailed investigation. Future experiments will focus on the cellular uptake, a potential release of the parent toxin through intestinal cleavage and the potential impact on inflammatory processes of DON-sulfates.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA