Pregled bibliografske jedinice broj: 770590
Generation of new mouse models for DNA damage response
Generation of new mouse models for DNA damage response // Phenomin European Advanced School for Mouse Phenogenomics
Alsace, Francuska, 2015. (poster, nije recenziran, neobjavljeni rad, ostalo)
CROSBI ID: 770590 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Generation of new mouse models for DNA damage response
Autori
Sabol, Maja ; Fricova, Dominika ; Kaspar, Petr ; Epp, Trevor Allen ; Beck, Inken Maria ; Sedlacek, Radislav
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, ostalo
Skup
Phenomin European Advanced School for Mouse Phenogenomics
Mjesto i datum
Alsace, Francuska, 08.06.2015. - 12.06.2015
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
DNA damage; gene editing nucleases; mouse model
Sažetak
Programmable nucleases are a method of choice for creation of mouse models where precision is required, for example in creating fluorescent fusion proteins under the control of the endogenous promoter to study protein function under physiological conditions in vivo. Most of the studies using fluorescently tagged proteins use expression vectors, and therefore create an unnatural overabundance of the protein, which may affect its activity. Here we present insertion of citrine gene after the ATG site of DNA damage response genes Fancd2 and 53BP1 using TALENs and CRISPRs. The correct integration was verified by southern blot, and functionality was demonstrated by induction of DNA damage in mouse embryonic fibroblasts.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
