Naslov
Local sensitivity analysis and metabolic control analysis of the biological part of the BTEX bioremediation model

Autori
Jurina, Tamara ; Jurinjak Tušek, Ana ; Čurlin, Mirjana

Izvornik
Biotechnology and bioprocess engineering (1226-8372) 20 (2015); 1071-1087

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
local sensitivity analysis ; metabolic control analysis ; bioremediation modelling ; BTEX

Sažetak
Environmental distribution and bioremediation of hydrocarbon pollutants is described in the literature with complex mathematical models. Better understanding and easier model application require detailed model analysis. In this work, local sensitivity analysis of the kinetic parameters and metabolic control analysis of the biological part of the integrated BTEX bioremediation model were performed. Local sensitivity analysis revealed that the dissolved oxygen concentration (SO) and particulate iron(III) oxide concentration (SFe) were the most sensitive to both positive and negative parameter value perturbations. In the case of model reactions, aerobic growth (r1) and aerobic growth on acetate (r13) were observed to be the most sensitive. The elasticity, flux control, and concentration control coefficients were estimated by applying the metabolic control analysis methodology. Metabolic control analysis revealed a positive effect of ammonium on all analysed model reactions. The results also indicated the importance of perturbation of the enzyme level catalysing iron reduction on acetate on model fluxes, as well as the importance of enzyme level catalysing aerobic growth on model metabolite concentration. The obtained results can be used in planning optimal operating strategy for BTEX bioremediation.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA