ࡱ> ?A>)bjbj .,rr $$$$$8888D| 8'0cccn&p&p&p&p&p&p&$O(+&E$\cc\\&$$&\$$n&\n&:,rۡP0j Z&&0' R++rr+$c>,$kccc&&^(ccc'\\\\+ccccccccc :Modulacija stani ne stijenke gljivi ne vrste Candida albicans berberinom Modulation of the cell wall of yeast Candida albicans with berberine I. Bobnjari1, J. Vlaini2, N. Zori3, I. Kosalec4 1University of Zagreb, Faculty of Pharmacy and Biochemistry, A. Kova ia 1, Zagreb, Croatia (student) 2Ruer Boakovi Institute, Division of Molecular Medicine, Laboratory of Molecular Neuropharmacology, Bijeni ka 54, Zagreb, Croatia 3Agency for Medicinal Product and Medical Devices, Ksaverska cesta 4, Zagreb, Croatia 4University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Microbiology, Schrottova 39/I, Zagreb, Croatia Candida albicans is endogenous opportunistic yeast that does not cause infections in healthy individuals but rather cause superficial to systemic infections in immuno-compromised individuals. The use of fungistatic drugs and the lack of -cidal drugs often results in strains that are not susceptible to commonly used antifungals, and/or display multidrug resistance (MDR). Therefore, there is a need to find new antifungals with novel targets. The aim of the present study was to determine the antifungal effects of the isoquinoline alkaloid berberine and to elucidate the mechanism of this effect in C. albicans. Using serial dilution assays we determined the minimal inhibitory concentration (MIC) of berberine against C. albicans. The ergosterol levels were assessed measuring the spectrophotometric absorbance profile between 240 and 300 nm (sterol quantitation method, SQM). To analyse the possible effects of berberine on the cell wall we tested the releasing of the crucial cell content using spectrophotometric measurement at 260/280 nm and 750 nm (corresponding to nucleic acids and proteins). Since secretion of aspartic proteinases is one of the important determinants associated with the pathogenicity of Candida species, we deterimened the effect of berberine on SAP2 activity using secreted aspartyl proteinase (SAP) test. In the present study, we observed that berberine affected cell growth of tested Candida albicans strain at the 17.75 g/ml indicating its good antifungal properties. In our study, the significant differences in mean ergosterol content were observed in berberine treated and non-treated group, which were dose and time dependent. Moreover, the results indicate that berberine and positive control (voriconazol) produce the same levels of ergosterol indicating their entanglement in the ergosterol biosynthesis. Moreover, berberine treatment caused the elevated levels of proteins and nucleic acids indicating the releasing of the cell content in the extracellular matrix. Namely, on the basis of these results we assumed that berberine treatment affects the cell membrane permeability and or integrity. 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