Naslov
Molecular characterization, functional and phylogenetic analysis of paralogous SSB proteins from Streptomyces coelicolor

Autori
Paradžik, Tina

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Poslijediplomski interdisciplinarni sveučilišni studij Molekularne bioznanosti

Mjesto
Osijek

Datum
06.09

Godina
2013

Stranica
133

Mentor
Vujaklija, Dušica

Ključne riječi
SSB; paralogous proteins; Streptomyces coelicolor; chromosome segregation; phylogeny

Sažetak
The linear chromosome of Streptomyces coelicolor contains two paralogous ssb genes, ssbA and ssbB. Mutational analysis of ssb genes indicated that ssbA is essential, whereas ssbB has a key role in chromosome segregation during sporulation. The two ssb genes were expressed differently, in accordance with their proposed biological roles. Localisation of fluorescently tagged SSB proteins was observed in the vegetative and aerial hyphae. Paralogous SSB proteins bind ssDNA with different affinity. Phylogenetic analysis of eubacterial SSBs indicated frequent duplication, loss of ssb genes and polyphyletic origin of well defined groups of SsbB proteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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