Naslov
Effects of herbaceous plant preparation of Cistanche on in vitro cytokine expression by rat osteoblasts

Autori
Caihong , Li ; Qing, Li ; Zhongjie, Liu ; Xiaoxu, Xing ; Dan, Tian ; Vrbanac, Zoran ; Smolec, Ozren, Stanin, Damir, Kos, Josip

Izvornik
Veterinarski arhiv. Supplement (0372-5480) 85 (2015), 3; 335-345

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
CISTHANCE; OSTEOBLASTS; OSTEOPROTEGRIN; RANKL; RAT
(OSTEOBLASTS; OSTEOPROTEGRIN; RANKL; RAT)

Sažetak
Osteoblasts (OB) play an important role in bone remodeling thro ugh the release of cytokines such as o s t e o p r o t e g e r i n osteoprotegerin ( ( O P G ) , a r e c e p t o r a c t i v a t o r o f n u c l e a r f a c t o r k a p p a - B l i g a n d OPG), a receptor activator of nuclear factor kappa-B ligand (RA NKL) and osteopontin (OPN). The objective of our study was to evaluate the effects o f Chinese herb extracts of Cistanche (Cistanche deserticola ) on the in vitro expression of these cytokines in rat OB cell culture. The prim ary OBs were isolated from the rat skull by trypsin and collagenase digestion. The ce llular phenotype was determined by the Swiss dyeing method, alkaline phosphatase staining, Von Kossa stainin g, and Alizarin red staining. The gene expression of OPG, RANKL and OPN were analyzed by quantitative real-time P CR. All treatments with herbaceous plant extracts of Cistanche increased the gene expression of OPG and RANKL at 72 hours of culturing, especially at concentrations of 5×10 -2 mg/mL, which were signifi cantly different compared to the control cultures. Treatment signifi cantly reduced the expression of the OPG/RANKL ratio at 48 hou rs and the expression of OPN mRNA at 72 hours of culturing. The in vitro exposure of OB to the plant preparation of Cistanche promoted the gene expression of OPG and RANKL and reduced the gene expression of OPG/RANKL and OPN, and thus, could maintain the balance of bone formation and resorption during bo ne metabolism

Izvorni jezik
Engleski

POVEZANOST RADA