Naslov
Validation of TLC-videodensitometric method for simultaneous determination of four isoflavones in herbal material

Autori
Fumić, Barbara ; Czarnik, Justyna ; Kwasniak, Konrad ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2nd Mediterranean Symposium on Medicinal and Aromatic Plants (MESMAP 2015) : book of Abstracts / Nazim, Sekeroglu ; Hakan, Cetinkaya - Ankara : Republic of Turkey Ministry of Forestry and Water Affairs General Directorate of Forestry, 2015, 581-581

Skup
Mediterranean Symposium on Medicinal and Aromatic Plants (2 ; 2015)

Mjesto i datum
Antalya, Turska, 22.04.2015. - 25.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
videodensitometry; TLC; isoflavones

Sažetak
Videodensitometry combined with (HP) TLC is gaining popularity due to very low cost of analysis and low toxicity to environment as compared to HPLC. Hereby we report validation of videodensitometric method for determination of four isoflavones in herbal material using Sorbfil software for chromatogram analysis. The method was validated by the assay tests of detection (LOD) and quantification limit (LOQ), calibration curve, repeatability and recovery. For chromatographic analysis, HPTLC Pre-Coated High performance Silica gel 60 F-254 plates (Merck, DE) were used. As mobile phase cyclohexane:dioxane:acetic acid (30:10:1 v/v/v) was employed. LOD and LOQ for daidzein, genistein, formononetin and biochanin A were 69.5, 64.6, 27.7and 25.0 ng respectively. The relationship between the concentration of standard solutions and the peak response was linear within the concentration range of 40 to 400 ng/spot for all four standards. Repeatability and recovery were 10.1-12.8% and 83-89%, respectively. The method was employed for analysis of isoflavon content in several plants from leguminosae family. The results showed that the tested chromatographic conditions, combined with videodensitometric analysis, were applicable for the simultaneous quantification of four isoflavones in herbal extracts.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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