Naslov
DESIGN OF OPTIMAL SOLVENT FOR EXTRACTION OF BIOACTIVE COMPOUNDS FROM LOTUS CORNICULATUS L.

Autori
Fumić, Barbara ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka- 5. Hrvatski kongres farmacije s međunarodnim sudjelovanjem / Zorc, Branka - Zagreb : Hrvatsko farmaceutsko društvo, 2015, 202-202

ISBN
987-953-7897-05-5

Skup
5. Hrvatski kongres farmacije s međunarodnim sudjelovanjem

Mjesto i datum
Rovinj, Hrvatska, 21.05.2015. - 24.05.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Lotus Corniculatus L.; RSM; phenolic compounds

Sažetak
Compounds with one or more phenolic groups are emerging as a potentially important new class of pharmaceutical leads with a broad range of biological activities. Recent interest in these substances has been stimulated by the results of the studies which indicate potential health benefits arising from the antioxidant activity of the phenolic structure. In this study, response surface methodology (RSM) was employed to optimize the extraction of phenolic compounds from aerial parts of Lotus corniculatus L., a common flowering plant from Fabaceae family Three parameters: ethanol concentration (%), extraction temperature (°C) and pH of extraction solvent, were optimized using the Box–Behnken design (BBD). Spectrophotometric methods were used for determination of phenolic compounds in the obtained extracts. Using the multiple regression analysis and analysis of variance (ANOVA), the experimental data were fitted to a second-order polynomial equation and were used to generate the mathematical model of optimization experiments. The optimal extraction conditions were as follows: 56 % of ethanol as extraction solvent, extraction temperature of 60 °C and pH of 8.5. Under these conditions, the extraction yields of Lotus corniculatus L. polyphenols, flavonoids and phenolic acids were 8.93, 0.46, and 0.07 mg/ml, respectively. These results were well matched with the predicted values (9.30 for polyphenols, 0.48 for flavonoids, and 0.08 for phenolic acids) thus indicating the suitability of RSM in optimizing the extraction of L. corniculatus aerial parts.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA