Naslov
Application of response surface methodology for extraction of antioxidative compounds from Medicago Sativa L.

Autori
Fumić, Barbara ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5. hrvatski kongres farmacije s međunarodnim sudjelovanjem : knjiga sažetaka / Zorc, Branka - Zagreb : Hrvatsko farmaceutsko društvo, 2015, 201-201

ISBN
978-9539897-05-5

Skup
Hrvatski kongres farmacije s međunarodnim sudjelovanjem (5 ; 2015)

Mjesto i datum
Rovinj, Hrvatska, 21.05.2015. - 24.05.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
RSM; antioxidant activity; Medicago Sativa L.

Sažetak
As dietary components, polyphenols are thought to have health-promoting properties due to their high antioxidant capacity, in both in vivo and in vitro systems. Functional hydroxyl groups of polyphenols mediate their antioxidant effects by scavenging free radicals and chelating metal ions. The chelation of metals could be crucial in the prevention of free radical generation which damage targeted biomolecules. In this study, the effect of extraction conditions on antiradical and chelating activity in Medicago sativa L. extracts was investigated. The optimum conditions for the extraction of compounds with high antioxidant activity from Medicago sativa L. were determined using response surface methodology (RSM). A three-factor, three-level Box-Behnken design with ethanol concentration (%), temperature (°C) and pH, as the independent variables, were selected for the study. Spectrophotometric methods were used for determination of responses, antiradical and chelating activity of the extracts. Data was analyzed using Design Expert software. The optimal conditions for extraction of antioxidant compounds were 40 % ethanol, 60°C and pH 7.2. Under these conditions, the experimental EC50 values for antiradical and chelating activity were 40.3 and 135.2 mg/ml, respectively. Experimental values agreed with the predicted ones, indicating good fitting of the model used and the success of response surface methodology for optimisation of extraction of antioxidant compounds from Medicago sativa L.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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