Naslov
Optimization of Cyclodextrin-assisted Extraction of Bioactive Compounds from Medicago Sativa L.

Autori
Fumić, Barbara ; Šimić, Sara ; Jablan, Jasna ; Inić, Suzana ; Jug, Mario ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstract / - , 2015, 15-15

Skup
1st International Congress of Controlled Release Society-Greek local chapter

Mjesto i datum
Atena, Grčka, 27.05.2015. - 28.05.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RSM; phenolic compounds; cyclodextrin

Sažetak
Purpose: In this study, the response surface methodology (RSM) was applied in order to optimize the cyclodextrin-assisted extraction of phenolic compounds from Medicago sativa L., as well as to optimize the antioxidant activity of the extracts prepared. Material and Methods: Box Behnken design was used to investigate the effects of three variables: concentration of (2-hydroxypropyl)-β-cyclodextrin (HPβCD) (mM), time of extraction (min) and ultrasonication strength (W) on the extraction of polyphenolic compounds and the antioxidative potential of the obtained extracts. Selected variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results. Phenolic content of the extracts was determined using Folin Ciocalteu reagent. The radical scavenging and chelating properties of the extracts were determined through reactivity with the stable 2, 2-diphenyl-1-picrylhydrazyl radical and ferrozine, respectivelly. Results were expressed as EC50 in mg of herbal material equivalent (HME) per mL. Data was analysed using Design Expert software. Results: The obtained results indicated that the optimal conditions for aqueous extraction of phenolic compounds were 25 mM HPβCD, 45 min, and 456 W. Extraction using 25 mM CD, 42 min and 760W gained extracts with simultaneously maximised antiradical and chelating activity. Extraction was repeated under optimum conditions to verify the validity of the model. The experimental value obtained for total polyphenols was 2.33 mg/mL, which was closely matched with the predicted value of 2.46 mg/mL. In addition, EC50 values for antiradical and chelating activity were 76.3 and 296.1 mg HME/mL, while predicted values were 80.6 and 294 mg HME/mLrespectively. Conclusion: Experimental values were in close agreement with those predicted, thus indicating suitability of RSM in optimizing the cyclodextrin-assisted extraction antioxidative compounds from M. sativa.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA