Naslov
Use of in vitro propagation of ‘Oblačinska’ sour cherry in the rootstock breeding

Autori
Dorić, Dušica ; Ognjanov, Vladislav ; Barać, Goran ; Ljubojević, Mirjana ; Pranjić, Ankica ; Dugalić, Krunoslav ; Ercisli, Sezai

Izvornik
Turkish journal of biology (1300-0152) 38 (2015); 2, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
dormant period ; elongation ; explant collection date ; Prunus cerasus L. ; rooting

Sažetak
Prunus cerasus L. ‘Oblačinska’ sour cherry germplasm was established in vitro directly from in situ plants on different explant collection dates, enabling quick clonal multiplication and its introduction to rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements and vitamins supplemented with (in mg l-1): 6–benzyladenine (BA) 0.5, indole–3–butyric acid (IBA) 0.01, gibberellic acid (GA3) 0.1, citric acid 10 and L–8 ascorbic acid . The lowest percent of contamination was noted in November and 9 December, when dormant buds were used as an explant source, and the highest with 10 actively growing shoot tips. Elongation phase of rosettes initiated from dormant buds remained major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. Increasing index of multiplication from 1.5 to 1.9

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

POVEZANOST RADA