Naslov
Evaluation of cationic nanosystems with melatonin using an eye- related bioavailability prediction model

Autori
Hafner, Anita ; Lovrić, Jasmina ; Duvnjak Romić, Marieta ; Juretić, Marina ; Pepić, Ivan ; Cetina-Čižmek, Biserka ; Filipović- Grčić, Jelena

Izvornik
European journal of pharmaceutical sciences (0928-0987) 75 (2015); 142-150

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cationic nano systems; Chitosan; Lecithin; Melatonin; Ocular drug delivery; Pluronic® F127

Sažetak
In this study, two types of nanosystems, namely lecithin/chitosan nanoparticles and Pluronic® F127/chitosan micelles, have been prepared and evaluated for their potential for the ocular delivery of melatonin, which is known to exert an ocular hypotensive effect. The melatonin content, particle size, zeta potential and in vitro drug release properties were studied as a function of the presence of chitosan in the nanosystem. Lecithin/chitosan nanoparticles were evaluated in terms of the mucoadhesive properties by a newly established method based on HCE-T cells, also used in in vitro biocompatibility and permeability studies.Lecithin/chitosan nanoparticles were significantly larger than the corresponding F127/chitosan micelles (mean diameter of 241.8 vs. 20.7. nm, respectively) and characterised by a higher surface charge (22.7 vs. 4.3. mV, respectively). The HCE-T cell viability assay did not show significant toxic effects of nanosystems investigated at the (relevant) chitosan concentration tested. The permeability study results confirmed the permeation enhancing effect of F127, which was hindered in the presence of chitosan. Lecithin/chitosan nanoparticles were characterised by prominent mucoadhesive properties and prolonged melatonin release, which was shown to control melatonin permeation across an in vitro corneal epithelial model. Such properties demonstrate the potential for nanoparticles to provide an extended pre-corneal residence time of melatonin, ensuring higher eye-related bioavailability and extended intraocular pressure reduction compared to melatonin in both aqueous and micelle solutions.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

Napomena
S.I.: Drug Delivery for Specific Populations.

POVEZANOST RADA