Naslov
Distribution of DNA-condensing protein complexes in the adenovirus core

Autori
J. Pérez-Berná, Ana ; Marion, Sanjin ; Javier Chichón, Francisco ; J. Fernández, José ; C. Winkler, Dennis ; L. Carrascosa, José ; C. Steven, Alasdair ; Šiber, Antonio ; San Martín, Carmen

Izvornik
Nucleic acids research (0305-1048) 43 (2015), 8; 4274-4283

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
adenovirus; DNA; cryo-EM; DNA-compacting proteins; core organization

Sažetak
Genome packing in adenovirus has long evaded precise description, since the viral dsDNA molecule condensed by proteins (core) lacks icosahedral order characteristic of the virus protein coating (capsid). We show that useful insights regarding the organization of the core can be inferred from the analysis of spatial distributions of the DNA and condensing protein units (adenosomes). These were obtained from the inspection of cryo-electron tomography reconstructions of individual human adenovirus particles. Our analysis shows that the core lacks symmetry and strict order, yet the adenosome distribution is not entirely random. The features of the distribution can be explained by modeling the condensing proteins and the part of the genome in each adenosome as very soft spheres, interacting repulsively with each other and with the capsid, producing a minimum outward pressure of ∼0.06 atm. Although the condensing proteins are connected by DNA in disrupted virion cores, in our models a backbone of DNA linking the adenosomes is not required to explain the experimental results in the confined state. In conclusion, the interior of an adenovirus infectious particle is a strongly confined and dense phase of soft particles (adenosomes) without a strictly defined DNA backbone.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

POVEZANOST RADA