Naslov
Lateral and transversal heterogeneity of human erythrocyte membrane

Autori
Žuvić-Butorac, Marta ; Schara, Milan ; Nemec, Marjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Supramolecular Strucutre and Function / Pifat-Mrzljak, Greta - Zagreb : Croatian Society of Natural Sciences, 1997, 153-153

Skup
Sixth International Summer School on Biophysics

Mjesto i datum
Rovinj, Hrvatska, 15.09.1997. - 27.09.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
cell membrane; EPR; lateral heterogeneity; transversal heterogeneity

Sažetak
Human erythrocytes were studied with respect to cell membrane organization and dynamics, as assessed by the spin probes methyl ester of doxyl palmitate, MeFASL(m, n). Electron paramagnetic resonance (EPR) line shapes were deconvoluted in order to determine the spectral components. Relative contribution of spectral components to the whole spectrum was interpreted in the sense of relative membrane population area (RMPA) by domains, characterized by different molecular ordering and dynamics in the lipid phase of cell membranes. At least three types of spectral components (domains), characterised by different order parameters, were resolved in spectra, indicating lateral heterogeneity of the membrane lipids with respect to the motional restriction imposed in the local membrane organization. As the spin label attached to the spin probe was moved deeper towards the membrane central region, the membrane organization changed. The change was obseved not only in the molecular order and dynamics of particular component, but also in the relative contribution of spectral components to the whole spectrum. The results were discussed and interpreted in the light of membrane lipid lateral and transversal organisation.

Izvorni jezik
Engleski

Znanstvena područja
Fizika

POVEZANOST RADA