Naslov
Spleen polar lipid content and fatty acid composition in the mice after feeding different MUFA and PUFA lipid supplemented diets

Autori
Milin, Čedomila ; Tota, Marin ; Ćuk, Mira ; Radošević-Stašić, Biserka ; Lisko, Dalibor ; Giacometti, Jasminka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
1st Croatian Congress on Molecular Life Sciences / Flogel-Mršić, Mirna ; Kućan, Željko - Zagreb : Pliva, 2002, 150-150

Skup
1st Croatian Congress on Molecular Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
polar lipids; spleen; dietary fats; vegetable oils

Sažetak
Monounsaturated oil (MUFA-diet) such as virgin olive oil (VOO) and polyunsaturated oil (PUFA-diet) such as corn oil (CO) are suggested to have selective physiological effects on membrane in tissues. The spleen polar lipid content and fatty acid composition were examined in 2-3 mo old Male Balb/c mice after three weeks of feeding lipid-supplemented diets containing olive oil (5% w/w, FOO group) and corn oil (5% w/w, FCO group). Total lipids were extracted according to Folch et al., and polar lipids were separated and purified by solid-phase extraction and analysed in form of the fatty acid methyl esters by gas chromatographic analysis. The results are shown as mean b standard deviation. Differences among groups were tested by nonparametric Kruskal-Wallis and Mann-Whitney U-test (p ƒŹ 0.05). Similar to total lipids, phospholipids were fatty acids affected by the type of dietary oils in examined tissue. Palmitic (16:0) and stearic (18:0) fatty acids were major saturated fatty acids (SFA) in polar lipids in the spleen tissue. In the FOO and FCO groups, the content of saturated fatty acids was lower than in the control group. In the FCO group, the content of unsaturated fatty acids was higher than in FOO group. Feeding lipid-supplemented diet reduced C20:4/C18:2 ratio in the FOO and FCO groups. The PUFA/MUFA and PUFA/SFA ratios (parameter which display a considerable degree of homeostasis in biomembranes) were lower in the FOO group and higher in FCO group than in control group. The oleic acid (C18:1n-9) content significantly increased in the MUFA diet than in the control and FCO group, and was the major monounsaturated acid in the spleen polar lipid tissue. Arachidonic acid (20:4n-6) was the major polyunsaturated acid in the spleen tissue sample, lower content of arachidonic acid was in FOO diet than in the control and FCO group. It is to conclude that the dietary enriched oils, type PUFA and MUFA affected the fatty acid composition in the total phospholipids of the spleen tissue.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti

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