Naslov
Pain responses in C57BL/6 cuprizone fed mice

Autori
Fenrich, Matija ; Milić, Jakov ; Muremi, Hendrik ; Zelen, Ada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
University of Pécs Medical School - Students' Research Conference 2014 / - Pečuh : Pécsi Tudományegyetem, 2014, 78-78

Skup
Medical School Pécs Students' Research Conference

Mjesto i datum
Pečuh, Mađarska, 03.04.2014. - 04.04.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
nociception; cuprizone; multiple sclerosis; demyelination

Sažetak
Pain responses in C57BL/6 cuprizone fed mice Introduction: Demyelination is a known cause of neuropathic pain in diseases such as multiple sclerosis (MS). Pathophysiology of central neuropathic pain in MS patients is considered to be a consequence of deterioration of myelin sheaths. This may be explained by abnormal generation of ectopic impulses as a response to demyelination and interruption of inhibitory transmission from the CNS. Feeding rodents with cuprizone leads to demyelination, which is used as a rodent model of MS. Aim: The aim of this study was to determine the differences in pain responses between healthy mice and cuprizone fed mice. Methods: The study was conducted on 12 six months old C57BL/6 mice, 6 of which were fed with 0.2% cuprizone powder chow diet for 20 days, and 6 were fed with standard chow. Pain sensitivity was tested using intraperitoneal application of 0, 8% ethanoic acid (visceral stimulus), von Frey's assay (mechanical stimulus), Hargreaves' plantar test (heat stimulus), and Acetone test (cold stimulus). Statistical analysis was done using Mann- Whitney U test. Results: Cuprizone fed mice showed significantly weaker responses to the von Frey assay (p=0.015) and the Acetone test (p=0.002) when compared to mice on normal diet. No significant differences between groups were observed when comparing the results of Hargreaves test (p=0.18) and intraperitoneal application of 0, 8% ethanoic acid (p=0.485). Conclusion: Our results suggest that demyelization primarily decreases the response to cold and mechanical stimuli, while the response to visceral and heat stimuli remains intact for longer periods of time.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA