Pregled bibliografske jedinice broj: 759439
Label Free Quantitation of CYP2C8, CYP2C9, and Two CYP4A11 Isoforms in Human Liver Microsomes
Label Free Quantitation of CYP2C8, CYP2C9, and Two CYP4A11 Isoforms in Human Liver Microsomes // VICB Student Research Symposium 2014
Nashville (TN), Sjedinjene Američke Države, 2014. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 759439 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Label Free Quantitation of CYP2C8, CYP2C9, and Two CYP4A11 Isoforms in Human Liver Microsomes
Autori
Albertolle, Matthew E. ; Bojic, Mirza ; Guengerich, Fred P.
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Skup
VICB Student Research Symposium 2014
Mjesto i datum
Nashville (TN), Sjedinjene Američke Države, 14.08.2014
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
cytochrome P450; LC; MS
Sažetak
Cytochrome P450s 4A11, 2C8, and 2C9 share arachidonic acid as a substrate with 4A11 forming 20-hydroxyeicosatetraeonic acid (20-HETE) and 2C8 and 2C9 forming epoxidation products, epoxyeicosatrienoic acids (EETs). Literature suggests that 20-HETE and EETs may play opposing autacoid roles in the vascular system. One 4A11 isoform, F434S, is associated with a higher incidence of hypertension. Using a mass spectrometry-based label free approach, we measured protein levels of both 2C8 and 2C9 as well as homozygotes and heterozygotes of 4A11 isoforms. Initial results suggest that F434S may be subject to differential post-translational regulation.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija, Farmacija
