Naslov
Impact of under-filled blood collection tubes containing K2EDTA and K3EDTA as anticoagulants on automated HbA1c testing

Autori
Krhač, Maja ; Radišić Biljak, Vanja ; Božičević, Sandra ; Vučić Lovrenčić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Clinical Chemistry and Laboratory Medicine (CCLM) / Plebani, Mario - , 2015, EA1-eA89

Skup
3rd Conference on Preanalytical Phase

Mjesto i datum
Porto, Portugal, 20.03.2015. - 21.03.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
under-filled blood collection tubes; K2EDTA; K3EDTA; HbA1c

Sažetak
Background: According to Clinical Laboratory Standard Institute (CLSI) guidelines and manufacturer’s regulation (Becton Dickinson), under-filled blood collection tubes may cause errors in laboratory testing resulting in potential misinterpretation of patient results. The aim of our study was to assess impact of under-filled blood collection tubes to HbA1c monitoring in patients with diabetes mellitus in two different anticoagulant tubes. Materials and methods: Blood samples from 29 patients were drowned into K2EDTA anticoagulant blood collection tubes with different volumes (4mL, as declared by the manufacturer, 2mL and 1mL) and tested for HbA1c levels. Specimens from another 26 patients sampled in K3EDTA anticoagulant tubes, underwent the same procedure. All samples were analyzed with an automated immunoturbidimetric procedure (TinaQuant-Integra 400Plus, Roche Diagnostics, USA). Statistical analysis was done using Deming regression in MedCalc 9.4.2.0 statistical software (MedCalc Software bvba, Mariakerke, Belgium). Results: There was no significant difference in group with K2EDTA anticoagulant between recommended volume of 4 mL blood and under-filled volume of 2 mL and 1 mL [regression equation (4mL and 2mL): y=0.5329+0.9282x, intercept A=0.5329, 95%CI=-0.08632 to 1.1520, slope B=0.9282, 95%CI=0.8382 to 1.0182 ; regression equation (4mL and 1mL): y=0.3180+0.9584x, intercept A=0.3180, 95%CI=-0.05893 to 0.6950, slope B=0.9584, 95%CI=0.9041 to 1.0127]. Results obtained from K3EDTA tubes were showing the same trend [regression equation (4mL and 2mL): y=0.02842+0.9900x, intercept A=0.02842, 95%CI=-0.3107 to 0.3675, slope B=0.9900, 95%CI=0.9449 to 1.0352 ; regression equation (4mL and 1mL): y=0.1177+0.9802x, intercept A=0.1177, 95%CI=-0.1253 to 0.3607, slope B=0.9802, 95%CI=0.9479 to 1.0126]. Although we found a slight deviation in bias for some results (± 0.3% NGSP/DCCT units), all of them were within established limits of clinical decision (± 0.5%). Conclusions: Despite general recommendations, under-filled K2EDTA or K3EDTA blood collection tubes have no impact on HbA1c level analysis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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