Naslov
Application of various Lactobacillus brevis cell disruption methods in order to obtain maximal alcohol dehydrogenase activity

Autori
Švarc, Anera ; Vasić-Rački, Đurđa ; Vrsalović Presečki, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Breath Analysis 2014 / Buszewski, B ; Kowalska, J - Toruń : Nicolaus Copernicus University, 2014, 147-147

Skup
Breath Analysis 2014

Mjesto i datum
Toruń, Poljska, 06.07.2014. - 09.07.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
alcohol dehydrogenase ; Lactobacillus brevis ; cell disruption ; EVOP

Sažetak
In this study, the cultivation of Lactobacillus brevis cells was carried out under anaerobic conditions in a batch reactor. The cell disruption process was performed to obtain the R- selective NADPH-dependent alcohol dehydrogenase (LbADH) in cell free extract. For that purpose five different cell disruption methods were tested. The combination of the biological and mechanical method proved to be the optimal L. brevis cell disruption method. To obtain the optimum conditions for L. brevis cell disruption two-level factorial design plans for two variables were set for three cell disruption methods. The results were statistically analyzed using evolutionary operations. The optimal method was the one where the cells were first lysed with lizozyme and then mechanically disrupted with glass beads in the ratio of 0, 05 g/g (mcells/mg.beads) during a 10-minute disruption time. The LbADH in cell free extract was used for biotransformation of acetophenone into (R)-1- phenylethanol in the presence of NADPH. The kinetics of LbADH in cell free extract for this reaction was measured and described by the double-substrate Michaelis- Menten model with included competitive product inhibition by NADP+. The kinetics of the reverse reaction was described by the second order kinetic. Based on the kinetic measurements, mathematical model was proposed and was validated in a batch reactor. The substrate equilibrium conversion in the batch reactor was 48, 4%.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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