Pregled bibliografske jedinice broj: 755656
Large-Scale Production and Protein Engineering of G Protein-Coupled Receptors for Structural Studies
Large-Scale Production and Protein Engineering of G Protein-Coupled Receptors for Structural Studies // Frontiers in Pharmacology, 6 (2015), 66-- doi:10.3389/fphar.2015.00066 (međunarodna recenzija, pregledni rad, znanstveni)
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Naslov
Large-Scale Production and Protein Engineering of G Protein-Coupled Receptors for Structural Studies
Autori
Milić, Dalibor ; Veprintsev, Dmitry B.
Izvornik
Frontiers in Pharmacology (1663-9812) 6
(2015);
66--
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni
Ključne riječi
G protein-coupled receptor (GPCR); Expression; purification; Protein Engineering; Isotope Labeling
Sažetak
Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular mechanisms of their action and contributed significantly to molecular pharmacology. This is primarily due to technical advances in protein engineering, production and crystallization of these important receptor targets. On the other hand, NMR spectroscopy of GPCRs, which can provide information about their dynamics, still remains challenging because of difficulties in preparation of isotopically labeled receptors and their low long-term stabilities. In this review, we discuss methods used for expression and purification of GPCRs for crystallographic and NMR studies. We also summarize protein engineering methods that played a crucial role in obtaining GPCR crystal structures.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
