Naslov
CYTO/GENOTOXIC EFFECT OF Frangula alnus Mil. BARK AND EMODIN

Autori
Domijan, Ana-Marija ; Gajski, Goran ; Gerić, Marko ; Radić Brkanac, Sandra ; Vujčić, Valerija ; Garaj-Vrhovac, Vera ; Kremer, Dario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Periodicum biologorum, Final programme and abstract book, Vol 116, Suppl 1, 2014, Third Meeting of the Croatian Association for Cancer Research, Zagreb. / - Zagreb, 2014, 42-42

Skup
Third Meeting of the Croatian Association for Cancer Research

Mjesto i datum
Zagreb, Hrvatska, 06.11.2014. - 07.11.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Frangula alnus; emodin; cyto/genotoxicity; antioxidant activity

Sažetak
Bark of Frangula alnus Mil. is widely used as laxative and can be found as component of herbal laxative preparations. Laxative property of F. alnus bark has been attributed to the presence of anthraquinones ; predominantly emodin. It is known that long-term use of anthraquinone-containing laxatives cause melanosis coli and it is suggested that they have possible role in the development of colorectal adenomas and cancer. However, studies on toxicity of bark of F. alnus are lacking. Therefore, present study investigated toxicity of F. alnus bark widely used as laxative. Human lymphocytes were treated with F. alnus bark extract or emodin, and cyto/genotoxicity and parameters of oxidative stress were evaluated. Moreover, polyphenol content of bark extract and antioxidant activity of the extract and emodin was measured by DPPH, ABTS and FRAP methods. The bark extract (500 μg/ml) induced cell death and DNA strand breaks, while level of reactive oxygen species (ROS) changed at 250 μg/ml. Emodin also induced cell death and DNA strand breaks at 150 μg/ml and 200 μg/ml, respectively, and the increase of ROS was observed at 25 μg/ml. These results suggest that both, bark extract and emodin, are cyto/genotoxic to human lymphocytes and that oxidative stress is involved in the mechanism of their toxicity. The results on antioxidant activity showed that, unlike emodin, bark extract possess moderate antioxidant capacity that can be related to relatively high phenolic content. Nevertheless, due to toxicological properties use of F. alnus bark and emodin-based herbal preparations should be taken with caution.

Izvorni jezik
Engleski

Znanstvena područja
Javno zdravstvo i zdravstvena zaštita

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