Naslov
Cloning the cDNA for murine U2 snRNP-A' gene and its differential expression in lymphocyte development

Autori
Antica, Mariastefania ; Kušić, Borka ; Hranilović, Dubravka ; Dietz, Al ; Vuk-Pavlović, Stanimir

Izvornik
Immunology Letters (0165-2478) 82 (2002), 3; 217-223

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
T lymphocytes; differentiation; thymus; U2 snRNP-A� gene; differential expression

Sažetak
Summary We studied genes differentially transcribed during development of murine thymocytes. By the use of differential display of mRNA by polymerase chain reaction (DD-PCR) we identified a cDNA for U2snRNP-A' from a transcript abundant in precursor thymocytes, but rare in mature T cells. The transcript was fully cloned and found to be 97% homologous to the human cDNA for U2 snRNP-A� . We found the gene most abundantly transcribed on day 15 of gestation and in adult prothymocytes, spleen, testis and liver. Further characterization of snRNP proteins in the mouse is warranted in an effort to establish animal models of autoimmunity relevant for studies of connective tissue diseases or systemic lupus erythematosus where patients harbor autoantibodies reactive to snRNP.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA