Naslov
Hormonal enhancement of insecticide efficacy in Tribolium castaneum : Oxidative stress and metabolic aspects

Autori
Plavšin, Ivana ; Stašková, Tereza ; Šerýa, Michal ; Smýkala, Vlastimil ; Hackenberger Kutuzović, Branimir ; Kodrík, Dalibor

Izvornik
Comparative biochemistry and physiology. C. Toxicology & pharmacology (1532-0456) 170 (2015); 19-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Adipokinetic hormone ; Insecticide ; RNA interference ; Metabolism ; Oxidative stress ; Mortality

Sažetak
Insect anti-stress responses, including those induced by insecticides, are controlled by adipokinetic hormones (AKHs). We examined the physiological consequences of Pyrap-AKH application on Tribolium castaneum adults (AKH-normal and AKH-deficient prepared by the RNAi technique) treated by two insecticides, pirimiphos-methyl and deltamethrin. Co-application of pirimiphos-methyl and/or deltamethrin with AKH significantly increased beetle mortality compared with application of the insecticides alone. This co-treatment was accompanied by substantial stimulation of general metabolism, as monitored by carbon dioxide production. Further, the insecticide treatment alone affected some basic markers of oxidative stress: it lowered total antioxidative capacity as well as the activity of superoxide dismutase in the beetle body ; in addition, it enhanced the activity of catalase and glutathione-S-transferase. However, these discrepancies in oxidative stress markers were eliminated/reduced by co-application with Pyrap-AKH. We suggest that the elevation of metabolism, which is probably accompanied with faster turnover of toxins, might be responsible for the higher mortality that results after AKH and insecticide co-application. Changes in oxidative stress markers are probably not included in the mechanisms responsible for increased mortality.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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