Naslov
Pathogenesis of Listeria monocytogenes infection following intra gastric inoculation in mice

Autori
Bubonja, Marina ; Dorić, Miljenko ; Abram, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian and Slovenian Symposium "Zoonoses today and tomorrow" / Prukenr-Radovčić, Estela; Presečki, Vladimir - Zagreb : Hrvatsko mikrobiološko društvo, 2001

Skup
Croatian and Slovenian Symposium on Microbiology and Infections Diseases "Zoonoses today and tomorrow"

Mjesto i datum
NP Plitvička jezera, Hrvatska, 21.06.2001. - 23.06.2001

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Listeria monocytogenes; intragastric

Sažetak
Listeria monocytogenes is a gram positive, facultative intracellular pathogen that is, in its severest form, responsible for meningoencephalitis in both animal and man. Listeria was recognized as a food-borne pathogen since the 1980s when several outbreaks of listeriosis were identified in Europe and North America. In an attempt to obtain a model resembling natural listeriosis, we studied the course of infection in BALB/c mice challanged by gastric intubation. Animals received 10(8) CFU of L. monocytogenes directly into the stomach after overnight fasting. Systemic infection and dissemination of Listeria to the lungs, liver, spleen and kidney was observed. The repeated intra-gastric administration of L. monocytogenes for 3 consecutive days led to development of severe liver failure. All infected animals exhibited continously high numbers of Listeria in their livers, severe pathohistological changes and extreme metabolic dissfunction according to high levels of serum aminotransferases. In addition, L. monocytogenes was isolated from the brains of all infected mice, even though histological examinations revealed no lesions. The increased severty of infection in mice challanged intra-gastrically by repeated doses, appeared to result from listerial multiplication in the livers and other organs rather than from local proliferation in the intestinal tract. This model seems to be potentially useful for research of the pathogenesis, predisposing factors and therapy in naturally introduced listeriosis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA