Naslov
COMPARATIVE PERFORMANCE OF FOUR METHODS FOR HIGH-THROUGHPUT GLYCOSYLATION ANALYSIS OF IMMUNOGLOBULIN G IN GENETIC AND EPIDEMIOLOGICAL RESEARCH

Autori
Pučić-Baković, Maja ; Huffman, Jennifer E ; Klarić, Lucija ; Hennig, Rene ; Selman, MHJ ; Aulchenko, Yurii ; Rapp, Erdmann ; Wuhrer, Manfred ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Glycobiology 24(11) / - , 2014

Skup
The 2014 Joint Meeting of the Society for Glycobiology and the Japanese Society for Carbohydrate Research

Mjesto i datum
Honolulu (HI), Sjedinjene Američke Države, 16.11.2014. - 19.11.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
IgG; glycomics; mass spectrometry; UPLC; high-throughput

Sažetak
The biological and clinical relevance of glycosylation is becoming increasingly recognized, leading to a growing interest in large-scale clinical and population-based studies. In the past few years, several methods for high-throughput analysis of glycans have been developed, but thorough validation and standardization of these methods is required before significant resources are invested in large-scale studies. In this study, we compared liquid chromatography, capillary gel electrophoresis, and two MS methods for quantitative profiling of N-glycosylation of IgG in the same data set of 1201 individuals. To evaluate the accuracy of the four methods we then performed analysis of association with genetic polymorphisms and age. Chromatographic methods with either fluorescent or MS-detection yielded slightly stronger associations than MS-only and multiplexed capillary gel electrophoresis, but at the expense of lower levels of throughput. Advantages and disadvantages of each method were identified, which should inform the selection of the most appropriate method in future studies

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA