Naslov
Detection of thiol modifications by hydrogen sulfide

Autori
Williams, E. ; Pead, S. ; Whiteman, M. ; , Wood, M.E. ; Wilson, I.D. ; Ladomery, M.R. ; Teklić, Tihana ; Lisjak, Miroslav ; Hancock, J.T.

Izvornik
Methods in enzymology (0076-6879) 555 (2015); 233-251

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
C. elegans ; hydrogen sulfide ; nitric oxide ; protein modification ; reactive oxygen species ; thiols

Sažetak
Hydrogen sulfide (H2S) is an important gasotransmitter in both animals and plants. Many physiological events, including responses to stress, have been suggested to involve H2S, at least in part. On the other hand, numerous responses have been reported following treatment with H2S, including changes in the levels of antioxidants and the activities of transcription factors. Therefore, it is important to understand and unravel the events that are taking place downstream of H2S in signaling pathways. H2S is known to interact with other reactive signaling molecules such as reactive oxygen species (ROS) and nitric oxide (NO). One of the mechanisms by which ROS and NO have effects in a cell is the modification of thiol groups on proteins, by oxidation or S- nitrosylation, respectively. Recently it has been reported that H2S can also modify thiols. Here we report a method for the determination of possible thiol modifications on proteins following the treatment with biological samples with H2S donors. Here, the nematode Caenorhabditis elegans is used as a model system but this method can be used for samples from animals or plants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

Napomena
S.I.: Hydrogen Sulfide in Redox Biology, Part B.

POVEZANOST RADA