Naslov
Acute changes of acethylcholinesterase activity and tau protein phosphorylation in mouse model of sporadic Alzheimer’s disease

Autori
Lončar, Andrija ; Knezović, Ana ; Šalković-Petrišić, Melita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Drugi simpozij Hrvatskog društva za znanost o laboratorijskim životinjama s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 10.10.2014

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
mice; streptozotocin; cholinergic; tau

Sažetak
Introduction: Intracerebroventricular (icv) administration of streptozotocin (STZ) generates a mouse model of sporadic Alzheimer’s disease. Long term effects of STZ-icv treatment have been well documented but the acute neurochemical impairment have not been investigated thoroughly enough. We aimed to explore acute (within 24 hours post icv injection) cholinergic and tau protein changes in STZ-icv mouse model. Materials and methods: Mice (strain C57Bl/6) were injected icv with STZ (1 and 1, 5 mg/kg) or vehicle-citrate buffer (controls). The animals were sacrificed 15 min, 1, 6 and 24 hours following the STZ-icv treatment. Acetylcholinesterase (AChE) activity in hippocampus (HPC) and parietal cortex (PC) was measured spectrophotometrically by Ellman’s method. Protein expression of phosphorylated tau protein (PHF13 and AT8) in HPC and PC was measured by SDS-PAGE electrophoresis, followed by Western blot analysis. Data were analysed by Mann-Whitney U test (p<0.05). Results: One hour after STZ-icv injection only the expression of PHF13 was found significantly increased in HPC (+26%) and PC (+37%), while the expression of AT8 was found significantly increased (+96%) in PC only. The AChE activity in PC was significantly increased 6 (+10%) and 24 hours (+15.5%) following the STZ-icv injection Conclusion: Results indicate that changes in tau phosphorylation are occurring earlier than those of AChE activity. Additionally, both changes occur earlier in PC than in the HPC, which indicates that STZ-icv administration triggers different acute post-treatment time- and brain-region dependent changes in the mouse brain.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA