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Pregled bibliografske jedinice broj: 737559

Studying lipid organization in biological membranes using liposomes and EPR spin labeling

Subczynski, Witold Karol; Raguz, Marija; Widomska, Justyna
Studying lipid organization in biological membranes using liposomes and EPR spin labeling // Methods in molecular biology, 606 (2010), 247-269 doi:10.1007/978-1-60761-447-0_18 (međunarodna recenzija, članak, znanstveni)

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Naslov
Studying lipid organization in biological membranes using liposomes and EPR spin labeling

Autori
Subczynski, Witold Karol ; Raguz, Marija ; Widomska, Justyna

Izvornik
Methods in molecular biology (1064-3745) 606 (2010); 247-269

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
liposomes, lipid bilayer, membrane domain, cholesterol, lens lipid, hydrophobic barrier fluidity, order, oxygen permeation, spin label, EPR

Sažetak
Electron paramagnetic resonance (EPR) spin- labeling methods provide a unique opportunity to determine the lateral organization of lipid bilayer membranes by discrimination of coexisting membrane domains or coexisting membrane phases. In some cases, coexisting membrane domains can be characterized without the need for their physical separation by profiles of alkyl chain order, fluidity, hydrophobicity, and oxygen diffusion- concentration product in situ. This chapter briefly explains how EPR spin-labeling methods can be used to obtain the above-mentioned profiles across lipid bilayer membranes (liposomes). These procedures will be illustrated by EPR measurements performed on multilamellar liposomes made of lipid extracts from cortical and nuclear fractions of the fiber cell plasma membrane of a cow-eye lens. To better elucidate the major factors that determine membrane properties, results for eye lens lipid membranes and simple model membranes that resemble the basic lipid composition of biological membranes will be compared.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija



POVEZANOST RADA

Ustanove:
Medicinski fakultet, Split

Profili:

Avatar Url Marija Raguž (autor)

Poveznice na cjeloviti tekst rada:

doi www.ncbi.nlm.nih.gov

Citiraj ovu publikaciju:

Subczynski, Witold Karol; Raguz, Marija; Widomska, Justyna
Studying lipid organization in biological membranes using liposomes and EPR spin labeling // Methods in molecular biology, 606 (2010), 247-269 doi:10.1007/978-1-60761-447-0_18 (međunarodna recenzija, članak, znanstveni)
Subczynski, W., Raguz, M. & Widomska, J. (2010) Studying lipid organization in biological membranes using liposomes and EPR spin labeling. Methods in molecular biology, 606, 247-269 doi:10.1007/978-1-60761-447-0_18.
@article{article, author = {Subczynski, Witold Karol and Raguz, Marija and Widomska, Justyna}, year = {2010}, pages = {247-269}, DOI = {10.1007/978-1-60761-447-0\_18}, keywords = {liposomes, lipid bilayer, membrane domain, cholesterol, lens lipid, hydrophobic barrier fluidity, order, oxygen permeation, spin label, EPR}, journal = {Methods in molecular biology}, doi = {10.1007/978-1-60761-447-0\_18}, volume = {606}, issn = {1064-3745}, title = {Studying lipid organization in biological membranes using liposomes and EPR spin labeling}, keyword = {liposomes, lipid bilayer, membrane domain, cholesterol, lens lipid, hydrophobic barrier fluidity, order, oxygen permeation, spin label, EPR} }
@article{article, author = {Subczynski, Witold Karol and Raguz, Marija and Widomska, Justyna}, year = {2010}, pages = {247-269}, DOI = {10.1007/978-1-60761-447-0\_18}, keywords = {liposomes, lipid bilayer, membrane domain, cholesterol, lens lipid, hydrophobic barrier fluidity, order, oxygen permeation, spin label, EPR}, journal = {Methods in molecular biology}, doi = {10.1007/978-1-60761-447-0\_18}, volume = {606}, issn = {1064-3745}, title = {Studying lipid organization in biological membranes using liposomes and EPR spin labeling}, keyword = {liposomes, lipid bilayer, membrane domain, cholesterol, lens lipid, hydrophobic barrier fluidity, order, oxygen permeation, spin label, EPR} }

Časopis indeksira:


  • Scopus
  • MEDLINE

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