Naslov
Bioorganic Research of Galactites tomentosa Moench. Honey Extracts: Enantiomeric Purity of Chiral Marker 3- Phenyllactic Acid

Autori
Jerković, Igor ; Roje, Marin ; Tuberoso, Carlo Ignazio Giovanni ; Marijanović, Zvonimir ; Kasum, Ana ; Obradović, Marina

Izvornik
Chirality (0899-0042) 26 (2014), 8; 405-410

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
volatile organic compounds ; GC-FID ; GC-MS ; chiral HPLC with polarimetric detector ; ( – )-3-phenyllactic acid

Sažetak
Thistle (Galactites tomentosa Moench.) honey organic extracts were obtained by headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE) and analyzed by gas chromatography (GC-FID and GC- MS) for the fi rst time. Most abundant headspace compounds were terpenes, particularly linalool derivatives (hotrienol was predominant with a range of 38.6 – 57.5%). 3-Phenyllactic acid dominated in the solvent extracts (77.4 – 86.4%) followed by minor percentages of other shikimate pathway derivatives. After determination of an adequate enantioseparation protocol on Chirallica PST-4 column, the honey solvent extracts were analyzed by high- performance liquid chromatography (HPLC). The chiral analysis revealed high enantiomeric excess (> 95%) of (–)-3-phenyllactic acid in all samples. Therefore, previous fi ndings of chemical markers of thistle honey were extended, providing new potential for advanced chemical fingerprinting (optical pure chemical marker).

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA