Pregled bibliografske jedinice broj: 734921
Expression of nestin in superior cervical ganglia of rats is influenced by gender and gonadectomy
Expression of nestin in superior cervical ganglia of rats is influenced by gender and gonadectomy // Journal of chemical neuroanatomy, 63 (2015), 6-12 doi:10.1016/j.jchemneu.2014.11.003 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 734921 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Expression of nestin in superior cervical ganglia of rats is influenced by gender and gonadectomy
Autori
Filipović, Natalija ; Mašek, Tomislav ; Grković, Ivica
Izvornik
Journal of chemical neuroanatomy (0891-0618) 63
(2015);
6-12
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
superior cervical ganglion; nestin; satellite cells; age; gender; gonadectomy
Sažetak
Neurons and glia arise from neural progenitor cells that express nestin. Although substantial changes in neuronal development were observed during the postnatal period, data concerning dynamics of nestin expression in the superior cervical ganglia (SCG) of rat during that period are lacking. It is known that gonadectomy and steroid hormones influence the development of neurons in the SCG during the postnatal period, but there are no data on how they influence the persistence of nestin expression in the SCG cells. The dynamics of nestin expression in the SCG, as well as the influence of gender and gonadectomy, was investigated in rats of three age groups. Three goups of male rats were sacrificed at 2, 3 and 6 months of age. Additional goups of male and female Sprague-Dawley rats were gonadectomized at the age of two months. After 30 days, they were sacrificed and SCGs were harvested and processed immunohistochemically. Immunoreactivity for nestin in the SCG was observed in satellite glia, based on their expression of s100. The proportion of neurons that were encircled with nestin-immunoreactive satellite cells (nestin encircled neurons, NEN) decreased between 2nd and 3rd month of age (p<0.05). The proportion of NEN was greater in the NPY+ than in the NPY- subpopulation. The proportion of NEN in the SCG of female rats was significantly higher (p<0.05) than that of both, the male rats and ovariectomised groups. The percentage of these neurons was significantly higher (p<0.05) in orchidectomised, in comparison to male rats. Results show the existence of nestin- immunoreactive satellite cells in the SCG of adult rats. A substantial decrease of nestin expression in SCG cells of rats, after the onset of sexual maturation, was observed. This decrease showed significant sex-dependence and was dramatically influenced by gonadal activity.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina
POVEZANOST RADA
Projekti:
216-2160528-0067 - Mehanizmi srčane boli u ishemiji, metaboličkim promjenama i u starosti (Grković, Ivica, MZOS ) ( CroRIS)
Ustanove:
Veterinarski fakultet, Zagreb,
Medicinski fakultet, Split
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
