Naslov
The genetic dependence of RecBCD-Gam mediated double strand end repair in Escherichia coli

Autori
Paškvan, Ivan ; Salaj-Šmic, Erika ; Ivančić-Baće, Ivana ; Zahradka, Ksenija ; Trgovčević, Željko ; Brčić-Kostić, Krunoslav

Izvornik
FEMS microbiology letters (0378-1097) 205 (2001), 2; 299-303

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
escherichia coli ; phage lambda ; RecBCD enzyme ; lambda-Gam protein ; recombination ; repair

Sažetak
The repair of double strand breaks after γ- irradiation in wild-type Escherichia coli lysogenic for λ cI857 red3 is more efficient when λ Gam protein is present. This phenomenon, called gam dependent radioresistance, requires the interaction of RecBCD enzyme and Gam protein. We compared cell survival after γ-irradiation in wild-type and mutant lysogens with and without induction of Gam by transient heat treatment of the cells (6 min, 42°C). The main conclusions are: (1) the RecBCD-Gam pathway of recombination repair is similar but not equivalent to RecBCD‡, a pathway operating in recD mutants ; (2) the RecBCD- Gam pathway is dependent on recJ, recQ and recN gene products and it is proposed that the RecBCD- Gam complex has ability to load RecA protein onto single strand DNA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA